acrylamide gel problem

"PBS::NELSONJ" at PBS.DFO.CA "PBS::NELSONJ" at PBS.DFO.CA
Wed Nov 15 15:23:07 EST 1995


Hi,
	We are trying to resolve ds DNA fragments in the 300 to 400 bp range.
We are using 7% acrylamide gels 19:1 and 1x tbe.  typically a run will be for
15 hrs overnight on a 16 cm gel.  These running conditions keeps smiling down
to a minimum but are problem is that the outer 8 lanes  are smeary.  The inner
ones are ok.  Does anyone have an idea of what might be up with these gels?
John
nelsonj at pbs.dfo.ca





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