We are trying to resolve ds DNA fragments in the 300 to 400 bp range.
We are using 7% acrylamide gels 19:1 and 1x tbe. typically a run will be for
15 hrs overnight on a 16 cm gel. These running conditions keeps smiling down
to a minimum but are problem is that the outer 8 lanes are smeary. The inner
ones are ok. Does anyone have an idea of what might be up with these gels?
nelsonj at pbs.dfo.ca