iandoc at aol.com
Wed Nov 15 11:06:05 EST 1995
Generally the best way is to use a fluorescent dye, assuming you have the
capability. BCECF (bis-Carboxyethylcarboxyfluorescein, if memory serves)
is probably one of the most widely used for this purpose. You can also
use any of a variety of other fluoresceins.
Yeast is trickier as they have a tendency to stuff the cell permeant forms
of these dyes into their vacuoles. I'm sure there are ways around this.
Try contacting one of the companies that specializes in Fluorescent
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