primers for TA cloning

celer at brno.ics.muni.cz celer at brno.ics.muni.cz
Wed Nov 15 01:06:57 EST 1995


Hi,
I can not find the information about first nucleotid in my primers which will
be used in TA cloning. I heard, that termostable polymerase is addind an A as
overhang depending of the first (or last) nucleotid in primer. Can you send me
this information?
Celer Vladimir
Veterinary univerzity Brno  Czech rep



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