PCR slippage

Christoph Baerwald c.baerwald at umds.ac.uk
Wed Nov 15 06:50:26 EST 1995


Hello,

I am sequencing PCR amplified genomic DNA containing a sequence of 11 - 
13 A in a row. Apparently in the PCR occurs a slippage which makes the 
sequence behind this sequel of A's unreadable. I have tried to sequence 
a shorter PCR fragment after optimizing the PCR conditions but the 
result was the same. 

Any help to overcome this problem is welcome.

Thank you very much in advance


Christoph
email:c.baerwald at umds.ac.uk





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