I am sequencing PCR amplified genomic DNA containing a sequence of 11 -
13 A in a row. Apparently in the PCR occurs a slippage which makes the
sequence behind this sequel of A's unreadable. I have tried to sequence
a shorter PCR fragment after optimizing the PCR conditions but the
result was the same.
Any help to overcome this problem is welcome.
Thank you very much in advance
email:c.baerwald at umds.ac.uk