BGHpA vs. SV40pA

Ralph Melcher melcher at mathematik.uni-marburg.de
Wed Nov 15 09:47:47 EST 1995


Hi netters,

I want to use the CMV-Promotor of pcDNA3 in another Vector that
contains a pac gene for selection in CHO-cells (pBEHpac18 (SV40
Promotor)).
It would be very easy to clone if I would only cut out the promotor
without the BGH polyA site. I would then use an SV40 polyA site with 
the CMV Promotor.
Does anyone know if that would lead to any problems ?


Please send me an E-Mail :

melcher at mathematik.uni-marburg.de

Sincerely,

Ralph Melcher
Institut für Physiologische Chemie, Marburg (AG Hasilik) 





More information about the Methods mailing list