Amicon tangential flow Q.

Sarah Tamara Braun brauns at rpi.edu
Wed Nov 15 08:20:03 EST 1995


Does anyone out there happen to concentrate seawater
using Amicon's hollow fiber tangential flow system?

If so, I use it to concentrate picoplankton (.2-200um) 
from 20L of seawater.  Yet the system doesn't work, the cells
seem to stick in the column, and
when we use TE to elute, we get only 66% recovery.

Any suggestions? I tried talking to Amicon, and all they
could suggest was coating the column with BSA, which
I intend to try.


Thanks in advance,


       Sarah Tamara Braun

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