Sequencing from E.coli.

Chandrasekhar N. Gujuluva cgujuluv at ucla.edu
Wed Nov 15 21:12:07 EST 1995


In article <487p0b$73n at highway.LeidenUniv.nl> bundock at rulsfb.leidenuniv.nl (Paul Bundockj) writes:
>From: bundock at rulsfb.leidenuniv.nl (Paul Bundockj)
>Subject: Sequencing from E.coli.
>Date: Mon, 13 Nov 95 16:40:53 PST

>Hi there, I'm trying to sequence ds plasmid DNA  rescued in the E.coli
>strain MC1061.So far its been very difficult to sequence mini prep DNA 
>although it should be possible if the prep is reasonably clean.This 
>problem with the E.coli background seems common, does anybody have a 
>list or reference for E.coli backgrounds and their suitability for 
>sequencing. By the way,I'm using T7 polymerase and 1.5-2 micro grams 
>template. Thanks a lot, Paul Bundock.





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