mdm-2 cDNA source?

Anton Scott Goustin asg at cmb.biosci.wayne.edu
Wed Nov 15 17:57:28 EST 1995


selby at lenti.med.umn.edu (Scott Selby (Med-Hem)) wrote:
>I'm looking for a source of mdm-2 cDNA.  I'd like to probe some Northern 
>blots, and haven't found a source yet.  Any help would be appreciated.
>
>______________________________________________________________________________
>    ||         Scott A. Selby               6098)o%:::%o(860
>   ====         Univ. of Minnesota          098)o%:::%o(8609
>   |  |__        Dept. of Med/Hematology     6o%:%o(86098)
>   |  |-.\        Box 480 UMHC                (86098)o
>   |__|  \\        Minneapolis, MN  55455   6098)o%::%o9  
>    ||   ||                                 098)o%::::::%o9
>  ======__|        selby at lenti.med.umn.edu   6o%::::::%o(860
> ________||__                                   6o%::%o(8609
>/____________\                                    o(86098)
>^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Scott,
Which mdm-2 cDNA do you need?  Mouse?  There are three human variants?
Did you try the mouse group who first cloned the cDNA:

Fakharzadeh, S.S., Trusko, S.P. & George, D.L.  (1991).  Tumorigenic 
   potential associated with enhanced expression of a gene that is
   amplified in a mouse tumor cell line. EMBO J.,  10(6),  1565-1569.

There are three mdm2 variants in humans:
Sigalas, I. & Lunec, J.  Multiple alternate spliced mdm2 transcripts 
   with loss of p53 binding domain sequences: transforming ability and 
   frequent detection in human cancer.  Unpublished.

Check out GenBank accession U033199.  If you need the human probe, just
made some custom oligonucleotides.





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