mdm-2 cDNA source?

[Anton Scott Goustin]

selby at lenti.med.umn.edu (Scott Selby (Med-Hem)) wrote:
>I'm looking for a source of mdm-2 cDNA.  I'd like to probe some Northern 
>blots, and haven't found a source yet.  Any help would be appreciated.
>
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>    ||         Scott A. Selby               6098)o%:::%o(860
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Scott,
Which mdm-2 cDNA do you need?  Mouse?  There are three human variants?
Did you try the mouse group who first cloned the cDNA:

Fakharzadeh, S.S., Trusko, S.P. & George, D.L.  (1991).  Tumorigenic 
   potential associated with enhanced expression of a gene that is
   amplified in a mouse tumor cell line. EMBO J.,  10(6),  1565-1569.

There are three mdm2 variants in humans:
Sigalas, I. & Lunec, J.  Multiple alternate spliced mdm2 transcripts 
   with loss of p53 binding domain sequences: transforming ability and 
   frequent detection in human cancer.  Unpublished.

Check out GenBank accession U033199.  If you need the human probe, just
made some custom oligonucleotides.