nuclear run-ons

[Mayumi Yagi]

Hi, Scott,
When I did nuclear run-ons, I routinely froze the isolated nuclei at -70o 
until I had enough samples to use up an entire order of UTP, and didn't 
notice any negative effects.  E-mail me if you want details.

Mayumi (myagi at u.washington.edu)
 On 16 Nov 1995, k wrote:

> Does anyone have any input into the affects of freezing cell pellets prior
> to preparing nuclei for nuclear run-ons or freezing the nuclei
> themselves.  Will this disrupt the nuclei and/or the initiation
> complexes?  Thanks
> 
> Scott Estes
> sde at mit.edu
> fax: 617 253-9863
> 
>