Gel purify 35S probe?
sfraser at acs5.acs.ucalgary.ca
Wed Nov 15 17:29:29 EST 1995
We've been using 35S-labelled RNA probes for RNase
protections. I have had no problems so far, except that I have
recently made a batch of probe that has a fair number of cleavage
breakdown products. Rather then throw out this batch, I would
like to be able to clean it up by gel purifying it. Is there
anyway to either visualize 35S-labelled RNA on PAGE, or can I
purify the probe from a gel that has been dried?
Thanks in advance,
Department of Biological Sciences
The University of Calgary
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