We are interested in removing fully-methylated plasmids from hemi-methylated
plasmids by DpnI digestion. Does anyone know an appropriate amount of
DpnI (in terms of units/ug) to use for this purpose? Most of our plasmids
are pUC based, and have approximately 15 DpnI sites. We suspect that
about 95% of our DNA is fully-methylated, so we are trying to eliminate
that background without cleaving the 5% that is hemi-methylated.