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Kent Wilcox kwilcox at POST.ITS.MCW.EDU
Thu Nov 16 16:37:47 EST 1995

We are interested in removing fully-methylated plasmids from hemi-methylated
plasmids by DpnI digestion. Does anyone know an appropriate amount of 
DpnI (in terms of units/ug) to use for this purpose? Most of our plasmids 
are pUC based, and have approximately 15 DpnI sites. We suspect that 
about 95% of our DNA is fully-methylated, so we are trying to eliminate 
that background without cleaving the 5% that is hemi-methylated. 

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