His tagging and 'cheaper' Nickel columns

[Richard A. Jefferson]

i all,

We are using His-6 tagging to purify many variants of mutagenized proteins for 
analysis, and are in need of an inexpensive (relatively) method to do so.   
The columns and column resins sold by Qiagen (Ni-NTA), Clontech (TALON), 
Invitrogen (Probond), tend to be very expensive.

Can someone recommend either an effective 'home-made' version of the Nickel 
chelate methods (and a source for the materials), or give an assessment of the 
relative merits of the above resins, and their re-useability?  

We will be purifying both secreted and cytoplasmic proteins, intially from E. 
coli.

Thanks in advance.

Richard


Richard A. Jefferson
Executive Director
CAMBIA
Center for the Application of Molecular Biology to International Agriculture
GPO Box 3200
Canberra ACT 2601,    Australia
tel (office):     +61 6 246 5302     tel (lab):       +61 6 246 5308
fax (office):    +61 6 246 5303     tel (home)    +61 6 235 9189  
Internet:	raj at cambia.org.au