We are using His-6 tagging to purify many variants of mutagenized proteins for
analysis, and are in need of an inexpensive (relatively) method to do so.
The columns and column resins sold by Qiagen (Ni-NTA), Clontech (TALON),
Invitrogen (Probond), tend to be very expensive.
Can someone recommend either an effective 'home-made' version of the Nickel
chelate methods (and a source for the materials), or give an assessment of the
relative merits of the above resins, and their re-useability?
We will be purifying both secreted and cytoplasmic proteins, intially from E.
Thanks in advance.
Richard A. Jefferson
Center for the Application of Molecular Biology to International Agriculture
GPO Box 3200
Canberra ACT 2601, Australia
tel (office): +61 6 246 5302 tel (lab): +61 6 246 5308
fax (office): +61 6 246 5303 tel (home) +61 6 235 9189
Internet: raj at cambia.org.au