Biorad Transblot Semi-Dry Transfer Cell

[Michael Myers]

In article <v01510100accff962c55f@[139.78.212.21]>,
wonderwench at BMB-FS1.BIOCHEM.OKSTATE.EDU (regina barnett) wrote:

>  Inquiry into possible solution(s) for cleaning problems with equipment.
>While using Biorad's Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell -
>(catalog # 170-3940), I've run into a spot.  The stainless steel cathode is
>forming a build up of precipitate which I assume is from the transfer
>buffer(s).  The cathode is cleansed with soft Kim-wipes and nanopure water,
>immediately upon end of transfer while it is still moist.  The buffer(s)
>have consisted of Glycine, Tris, Methanol, Water & +/- AHA.  The Biorad
>people say that they have never heard complaint of this problem, and
>suggested soaking filter papers with water and placing on the areas in
>question for ~ 3 hours.  This seems to have gotten me nowhere.  Any
>suggestions??  Any others out there with similar problems with the same
>unit??  Thanks!

We see the same build-up. I can't say whether it's bad for the metal over
time, but 10% acetic acid is effective at removing the residue. Just rinse
well with water when done.

Michael Myers
Laboratory of Genetics
The Rockefeller University
212-327-8233  (-7420 fax)
myersm at rockvax.rockefeller.edu