IPCR and Diff. Display help

Carlyn S. Buckler ckeith at biosci.mbp.missouri.edu
Thu Nov 16 11:25:39 EST 1995


Hello -
        Has anyone out there used a protocol written up by Souer, et al.,
(from "A general method to isolate genes tagged by a high copy number
transposable element", E. Souer, et al, The Plant Journal, 1995
7:677-685)?  It uses a combination of inverse polymerase chain reaction
and differential screening of amplification products to isolate
transposable element flanking sequences unique for mutant plants.  It also
uses a "vectorette" system - anyways, it sounds really slick, and would
help to avoid a million Southerns, but there are a couple holes in the
protocol (can't get a hold of the folks, are at Vrije Universiteit,
Amsterdam).

Thanks,
Carlyn S.Buckler

-- 
Carlyn Buckler



More information about the Methods mailing list