nuclear run-ons

[Cam Patterson]

In Article <mit.edu-1611950909340001 at cancer-ctr-mac-39.mit.edu>, mit.edu (k)
wrote:
>Does anyone have any input into the affects of freezing cell pellets prior
>to preparing nuclei for nuclear run-ons or freezing the nuclei
>themselves.  Will this disrupt the nuclei and/or the initiation
>complexes?  Thanks
>
>Scott Estes
>sde at mit.edu
>fax: 617 253-9863
Scott,
Our lab routinely freezes down pellets by immediately immersing them in
liquid nitrogen once the pellet is resuspended with no loss of efficiency of
transcription. Good luck.
cam