nuclear run-ons

Cam Patterson patterson at
Thu Nov 16 05:47:48 EST 1995

In Article < at>, (k)
>Does anyone have any input into the affects of freezing cell pellets prior
>to preparing nuclei for nuclear run-ons or freezing the nuclei
>themselves.  Will this disrupt the nuclei and/or the initiation
>complexes?  Thanks
>Scott Estes
>sde at
>fax: 617 253-9863
Our lab routinely freezes down pellets by immediately immersing them in
liquid nitrogen once the pellet is resuspended with no loss of efficiency of
transcription. Good luck.

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