In Article <mit.edu-1611950909340001 at cancer-ctr-mac-39.mit.edu>, mit.edu (k)
>Does anyone have any input into the affects of freezing cell pellets prior
>to preparing nuclei for nuclear run-ons or freezing the nuclei
>themselves. Will this disrupt the nuclei and/or the initiation
>sde at mit.edu>fax: 617 253-9863
Our lab routinely freezes down pellets by immediately immersing them in
liquid nitrogen once the pellet is resuspended with no loss of efficiency of
transcription. Good luck.