gaughan at bms.com
Fri Nov 17 13:58:34 EST 1995
In article <1995Nov15.163448 at molbiol.ox.ac.uk>, tflanigan at molbiol.ox.ac.uk
> In article <1995Nov15.162935 at molbiol.ox.ac.uk>,
tflanigan at molbiol.ox.ac.uk writes:
> RE: sequencing gels
> I hope someone can help! We silanise one plate of the sequencing apparatus
> with sigmacote and it's ben working well. We now seem to have what
> be a build-up of silane on this plate and the gel seems to be sticking to it.
> We use decon as the detergent to clean the apparatus and use sigmacote
> everytime we pour a gel. Is this problem silane or something else? If
> silane, can anyone suggest a better detergent than decon? Is it
> we're using the sigmacote TOO OFTEN??!!
> Thanks in advance,
> Tom tflanigan at molbiol.ox.ac.uk
Build-up can be remove w base (e.g. soak in 0.5 M NaOH a few hrs) The descibed
problem can also occur as a result of 'migration' of the silane to the opposing
plate (2 slippery plates = gel can't decide who to stick to). Minimize amnts
of silanizing agent; don't leave plates in apposition longer than nec; base wash
your plates often.
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