Thiols in KLH for conjugation

Keld Sorensen KeldS at uic.edu
Fri Nov 17 11:18:13 EST 1995


Matt Santo <babco at crl.com> wrote:
>I am interested in conjugating a hapten to KLH through the
> thiol groups in KLH.  Does anyone know how many thiol groups
>are availble for conjugation?

I don't think you have ANY free thiols. You can make
about 200 or so with Traut's reagent or SATA (both
from Pierce) - 

Keld.





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