glassmilk

nsaunders at molbiol.ox.ac.uk nsaunders at molbiol.ox.ac.uk
Fri Nov 17 13:30:47 EST 1995


Keeps cropping up this one; a photocopy just arrived in our lab called "an 
inexpensive alternative to Glassmilk for DNA purification", it's Trends In 
Genetics 11:pg 8, the technical tips section. Apparently you use silica from 
Sigma (S-5631), wash it in PBS and resuspend in NaI. Anyway it's all there.
They also describe a wash buffer which is presumably similar to NewWash, that 
other old chestnut: 50mM NaCl, 10mM Tris HCl pH7.5, 2.5mM EDTA, 50% v/v 
ethanol.

Have fun,

Neil

nsaunders at molbiol.ox.ac.uk




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