protein pptn in acetone problem

brett brett at BORCIM.WUSTL.EDU
Fri Nov 17 13:42:04 EST 1995


Hello, I'm trying to ppt. my protein using cold acetone. It was in a 50uM
sodium phosphate buffer. I added 8 vol. acetone, and now have a large,
chunky ppt. I believe the large chunks are phosphate, as buffer alone gave
the
same appearance. Parallel ppt's of the same protein in a citrate buffer
gave a finer, milky ppt. I now want to dry down the pellets and resuspend
them for SDS-PAGE analysis, but am worried about the salt. Anyone have any
suggestions for what to do? I thought I might resuspend the chunky pellets,
dialyze into another buffer, and reppt. Thanks in advance,



Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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