Its common knowledge that taq breaksdown throughout the PCR reaction.
Is it like a dimer for instance that breaks down into subunits which
have no enzyme activity, so that at any one stage in the PCR there are
two sorts of molecules some fully active and others with no activity?
Do the individual taq molecules slow down by some modification of their
structure which doesnt turn off their activity completely in the one
hit, like a light switch, but is rather like a dimming switch in that
activity is lost gradually over time?
Thanks for any answers and many thanks if you include references.
hjs at renal.wh.su.edu.au