Stephen Lessnick <stevel at ucla.edu> wrote:
>>I expose my 35S sequencing gels anyway at RT, because Idon't like to wait
>>for thawing the next morning :-)
>>>A couple of points...
>>1) Why would one expose 35S sequencing gels at -70 degrees in the first
>place since they don't possess enough energy to make the enhancing screen
>worthwhile. On the other hand, soaking the gel in salicylate or PPO
>would provide the enhancement effect, and then I believe it would make a
>difference to expose the gel at -70.
>>2) You don't really need to thaw the gel after a -70 degree exposure.
>Just take the frozen cassette into the darkroom, and pull the film off
>while frozen. Provided the X-omat is properly warmed up, the autorad
>will come out just fine. I know, because I usually had to develop my
>films immediately before our a.m. lab meetings...
I do not believe that the -70C exposure has anything to do with
fluors or intensifying screens. The -70C exposure helps to reduce
reciprocity failure in the film in a similar way to preflashing
of x-ray film does. As I understand it a silver halide grain
must be 'hit' several times within a given time period to be
activated. At room temp the half-life between activating 'hits'
is very short. At -70C the window for receiving the activation
events (photons or beta particles) is much longer thus the film
has a much more linear response to low levels of activity.
// \\ // \\ // \\ // \\ // \\ // \\ // \\
Eric Hugo, Ph.D.// |:,\\': | \\ // | :,\\': | \\ // | :,\\': | \\
e_hugo at dsu1.dsu.nodak.edu\ | | \\ // | | // \\ | | \\ // | | // \\ | |
Asst. Professor, Biology \\ | :,\\': | // \\ | :,\\': | // \\ |
Dickinson State University \\ // \\ // \\ // \\ // \\
Dickinson, ND 58601 |PGP 2.6 Key available from most key servers