SV40 polyadenylation

Clinton MacDonald cbbccm2 at ttuhsc.edu
Fri Nov 17 16:39:57 EST 1995


Bernard:

In article <1995Nov17.203327.20763 at alw.nih.gov>, bernard at elsie.nci.nih.gov
(Bernard Murray) wrote:

> BOTTOM LINE: For the usual (eg. 369 bp) SV40 polyadenlation sequence
> hom much of the actual sequence appears in the mature mRNA?
>[...]
> There are two AAUAAA sequences in the SV40 signal but I don't know
> which of these (or both) are used.

Actually, depending on which strand you are considering, there are two
AAUAAAs on what is known as the "early" strand and one on what is known as
the "late" strand. For the late strand, the sequence would be:

   [BamHI]...*AATAAA*CAAGTTAACAACA..ACAATTGC...[BclI]

with the ".." indicating the cleavage site (poly(A) starts there). The
early strand goes like:

   [BclI]...ACA*AATAAA*GCATTTTTTTCACTGCA..TTCTAG...[BamHI]

(showing only the proximal of the two AATAAAs).

   For more than you would ever care to know about SV40 poly(A) sites see
MacDonald et al., Mol. Cell. Biol. 14,6647-6654, 1994.

   If that is not enough information, feel free to e-mail me. Good luck!

   Clint

-- 
\ Dr. Clinton C. MacDonald   \ cbbccm2 at ttuhsc.edu (preferred)
 \ Cell Biology & Biochemistry\ cbbccm at wpoffice.net.ttuhsc.edu
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