His tagging and 'cheaper' Nickel columns

Curt Ashendel ashendel at aclcb.purdue.edu
Fri Nov 17 16:01:20 EST 1995


On 17 Nov 1995 16:19:19 GMT, 
Keld Sorensen   <KeldS at uic.edu> wrote:

>Pierce has IDA Sepharose at a much lower price and good performance.

Sigma also sells IDA (imminodiacetic acid) sepharose for a reasonably 
low price. But I was under the impression that IDA's affinity 
for Ni++ was too low  and there would be a problme with His-6 proteins 
bleeding off the column during loading or washing. Does it really work 
as well as NTA-agarose?


Curt Ashendel
Purdue University
West Lafayette, IN
ashendel at aclcb.purdue.edu



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