antibody affinity purification

Pierce Chemical Co PierceChem at
Wed Nov 15 15:52:40 EST 1995

To purify general polyclonal IgGs from rabbit serum, use an immobilized protein A column.  It 
has as good or better binding than protein G or A/G and they are cheaper.  If your purifying 
monoclonals by using its antigen, then immobilize that antigen on an oxidised agarose matrix and 
then pass the serum through.

Technical Assistance
Pierce Chemical Co
Phone: 1-815-968-0747   
Fax: 1-815-968-7316  
E-mail: PierceChem at

More information about the Methods mailing list