antibody affinity purification

Pierce Chemical Co PierceChem at
Wed Nov 15 15:50:14 EST 1995

To purify rabbit IgGs from serum use a protein A column.  It has as good or better binding to 
rabbit IgG than protein G or A/G and is cheaper.  If you're purifying monoclonals, attach the 
antigen to an oxidized agarose matrix and use that to capture your specific antibody.
Tom Brotcke

Technical Assistance
Pierce Chemical Co
Phone: 1-815-968-0747   
Fax: 1-815-968-7316  
E-mail: PierceChem at

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