primers for TA cloning

John Dixon jpcd0 at mole.bio.cam.ac.uk
Fri Nov 17 07:09:41 EST 1995


> 
> Taq polymerase adds a 3' A independently of the PCR product you have
> amplified. The primers used make no difference, but be sure to ligate to
> the TA vector immediately following amplification to avoid degradation of
> the A overhangs.
> 
> good luck,
> Bill Cook
> Northwestern

Does anyone know of a ref about this loss of A overhang business? Will all
overhangs be degraded? How soon are they lost if frozen? etc etc

Any info appreciated

cheers 

Johnny D

-- 
John Dixon                                        Lab 44 (1223) 334131
Wellcome/CRC Institute                            Fax 44 (1223) 334134
Department of Genetics
Cambridge University    
United Kingdom                           e-m: jpcd0 at mole.bio.cam.ac.uk



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