pouring sequencing gels

jim hartley jhartley at access2.digex.net
Fri Nov 17 06:23:53 EST 1995


I too vote for pouring gels flat, no tape, both ends open.  It's really 
easy, nothing to leak, you can tap on the glass if the liquid encounters 
a dirty spot.

On Wed, 15 Nov 1995 futers at biovax.leeds.ac.uk wrote:

> In article <1995Nov15.110758 at molbiol.ox.ac.uk>, nsaunders at molbiol.ox.ac.uk writes:
> >Yes, what is the "usual way" to pour a sequencing gel. We have the simplest 
> >method of all, judging by what I've read so far; tape the sides and bottom, 
> >clamp the sides with bulldog clips, mix the gel in a conical flask and just 
> >tip it in! Well, not quite 'just tip'; you hold the plates by the bottom 
> >corner with one hand and tuck the opposite corner into your armpit, hold it at 
> >about 45 degrees and sloping away from you and pour slowly into one corner. 
> >The mix flows down one edge, across the bottom and rises up to fill the gel, 
> >any bubbles flow along this path and quickly rise to the top and burst. Then 
> >just gently lay it down and pop the combs in at the top. And you need nice, 
> >clean plates, goes without saying. Ours are scrubbed with detergent, rinsed, 
> >wiped thoroughly with alcohol and dried. 
> >
> >I guess it's a bit hard to describe without seeing it; point is, there's a lot 
> >of fancy bits and pieces in molecular biology that noone actually needs.
> >
> >Neil
> >
> >nsaunders at molbiol.ox.ac.uk
> 
> An easier method is to lay the plates flat on a support such as an
> empty tip rack.  The plates are held together by just two bulldog
> clips, and no tape.  Make sure the plates are horizontal, pour the 
> acrylamide in at the comb end and let the plates fill by capillary 
> action.  If a bubble starts to form, just tap the plate just above
> the forming bubble, but this is unneccessay if the plates are clean.
> 
>                  Simon Futers (futers at biovax.leeds.ac.uk)
>  
> 
> 



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