pouring sequencing gels
jhartley at access2.digex.net
Fri Nov 17 06:23:53 EST 1995
I too vote for pouring gels flat, no tape, both ends open. It's really
easy, nothing to leak, you can tap on the glass if the liquid encounters
a dirty spot.
On Wed, 15 Nov 1995 futers at biovax.leeds.ac.uk wrote:
> In article <1995Nov15.110758 at molbiol.ox.ac.uk>, nsaunders at molbiol.ox.ac.uk writes:
> >Yes, what is the "usual way" to pour a sequencing gel. We have the simplest
> >method of all, judging by what I've read so far; tape the sides and bottom,
> >clamp the sides with bulldog clips, mix the gel in a conical flask and just
> >tip it in! Well, not quite 'just tip'; you hold the plates by the bottom
> >corner with one hand and tuck the opposite corner into your armpit, hold it at
> >about 45 degrees and sloping away from you and pour slowly into one corner.
> >The mix flows down one edge, across the bottom and rises up to fill the gel,
> >any bubbles flow along this path and quickly rise to the top and burst. Then
> >just gently lay it down and pop the combs in at the top. And you need nice,
> >clean plates, goes without saying. Ours are scrubbed with detergent, rinsed,
> >wiped thoroughly with alcohol and dried.
> >I guess it's a bit hard to describe without seeing it; point is, there's a lot
> >of fancy bits and pieces in molecular biology that noone actually needs.
> >nsaunders at molbiol.ox.ac.uk
> An easier method is to lay the plates flat on a support such as an
> empty tip rack. The plates are held together by just two bulldog
> clips, and no tape. Make sure the plates are horizontal, pour the
> acrylamide in at the comb end and let the plates fill by capillary
> action. If a bubble starts to form, just tap the plate just above
> the forming bubble, but this is unneccessay if the plates are clean.
> Simon Futers (futers at biovax.leeds.ac.uk)
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