His tagging and 'cheaper' Nickel columns
mbzspd at granby.nott.ac.uk
Fri Nov 17 03:36:09 EST 1995
One place you might want to look if you don't want to make your own NTA
Agarose (or whatever) is Pharmacia. They now sell Chelating Sepharose FF
(Cat. No: 17-0575-01 for 50ml pack size) which chelates ANY metal ion
(more or less) that you want and comes uncharged i.e. you can charge the
resin as you want. It's _much_ cheaper (I think 50ml is ~80 pounds
sterling) than the pre-charged ripoff resins from Invitrogen etc.
Hope thats of some use,
Dr. Simon Dawson TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry FAX:+44 (0)115 9422225
Queens Medical Centre Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
U.K. "Back off man, I'm a scientist!" - Bill Murray.
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