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His tagging and 'cheaper' Nickel columns

Simon Dawson mbzspd at granby.nott.ac.uk
Fri Nov 17 03:36:09 EST 1995

   One place you might want to look if you don't want to make your own NTA 
Agarose (or whatever) is Pharmacia. They now sell Chelating Sepharose FF 
(Cat. No: 17-0575-01 for 50ml pack size) which chelates ANY metal ion 
(more or less) that you want and comes uncharged i.e. you can charge the 
resin as you want. It's _much_ cheaper (I think 50ml is ~80 pounds 
sterling) than the pre-charged ripoff resins from Invitrogen etc.
  Hope thats of some use,



Dr. Simon Dawson       TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry  FAX:+44 (0)115 9422225
Queens Medical Centre  Email:Simon.Dawson at .nott.ac.uk
Clifton Boulevard      http://www.ccc.nottingham.ac.uk/~mbzspd/Simon.html
U.K.                   "Back off man, I'm a scientist!" - Bill Murray.

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