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Non-IgG Anti-BrdU antibody?

Charles Murry murry at u.washington.edu
Sat Nov 18 22:47:11 EST 1995

I am currently attempting to perform double label immunocytochemistry, 
using antibodies to bromodeoxyuridine (BrdU) and various myosin heavy 
chains.  Currently, all of the reagents I have are mouse monoclonal 
IgG's.  I am getting cross-reaction between my first and second 
antibodies, despite numerous attempts to block reactivity.

Does anyone know of a non-mouse IgG anti-BrdU antibody that would be 
useful for staining nuclei in proliferating cells?  It would be very 
useful to have an IgM or a rabbit/goat/etc. polyclonal for double labeling.

Thank you very much.

Chuck Murry
Department of Pathology
Box 357335
University of Washington
Seattle, WA 98195
phone (206) 543-7483
fax   (206) 685-3662
murry at u.washington.edu

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