I am currently attempting to perform double label immunocytochemistry,
using antibodies to bromodeoxyuridine (BrdU) and various myosin heavy
chains. Currently, all of the reagents I have are mouse monoclonal
IgG's. I am getting cross-reaction between my first and second
antibodies, despite numerous attempts to block reactivity.
Does anyone know of a non-mouse IgG anti-BrdU antibody that would be
useful for staining nuclei in proliferating cells? It would be very
useful to have an IgM or a rabbit/goat/etc. polyclonal for double labeling.
Thank you very much.
Department of Pathology
University of Washington
Seattle, WA 98195
phone (206) 543-7483
fax (206) 685-3662
murry at u.washington.edu