(no subject)

[Graham Hamilton]

I would be very interested in hearing from anyone who has had experience 
using the Gibco/BRL Elongase PCR kit (containing pyrococcus thermostable 
polymerase and Taq). I have just tried it out to amplify a ~12 kb 
product. However, I could not see any bands at all, apart from primers. 
I'm using  30-mer degenerate oligos. Also the primers contain inosine. 
The PCR conditions were those recommended by the kit. Annealing 
temperature of 55 C was used, which is known to give a range of shorter 
non-specific products using Taq alone.