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salt-gradient sequencing gels

James Tsuruta jtsuruta at med.unc.edu
Mon Nov 20 13:08:25 EST 1995

How are salt-gradient sequencing gels run?  I saw an old post that said:

0.5X TBE in upper resevoir
1X TBE in lower resevoir + "3M NaOAC"

I'm assuming that the concentration of acetate in the lower resevoir is
less than 3Molar. (maybe 0.3M?) Can anyone tell me how they do this?

Thanks in advance.

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