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How are salt-gradient sequencing gels run? I saw an old post that said: 0.5X TBE in upper resevoir 1X TBE in lower resevoir + "3M NaOAC" I'm assuming that the concentration of acetate in the lower resevoir is less than 3Molar. (maybe 0.3M?) Can anyone tell me how they do this? Thanks in advance.