IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Pfu DNA polymerase (change Tanneal too)

Jody K. Hirsh jkh141 at nwu.edu
Mon Nov 20 16:47:12 EST 1995

In article <48nla9$2go at news.duke.edu>, vando005 at mc.duke.edu says...

>I posted a reply just a couple of days ago on PFU polymerases
>proofreading. The only thing you have to make sure with this
>enzyme is to use it in its OWN buffer, the composition is different
>than a regular TAQ buffer.Hope this helps, good luck,
Also you will need to lower the annealing temperature about 5 degrees or 
so because of the change in the salt conc. with the Pfu buffer!!!
Jody K. Hirsh
Northwestern University, Chicago, IL.   USA
jkh141 at nwu.edu

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net