In article <48nla9$2go at news.duke.edu>, vando005 at mc.duke.edu says...
>>I posted a reply just a couple of days ago on PFU polymerases
>proofreading. The only thing you have to make sure with this
>enzyme is to use it in its OWN buffer, the composition is different
>than a regular TAQ buffer.Hope this helps, good luck,
Also you will need to lower the annealing temperature about 5 degrees or
so because of the change in the salt conc. with the Pfu buffer!!!
Jody K. Hirsh
Northwestern University, Chicago, IL. USA
jkh141 at nwu.edu