Pfu DNA polymerase (change Tanneal too)

Jody K. Hirsh jkh141 at nwu.edu
Mon Nov 20 16:47:12 EST 1995


In article <48nla9$2go at news.duke.edu>, vando005 at mc.duke.edu says...
>


>Elena,
>
>I posted a reply just a couple of days ago on PFU polymerases
>proofreading. The only thing you have to make sure with this
>enzyme is to use it in its OWN buffer, the composition is different
>than a regular TAQ buffer.Hope this helps, good luck,
>
>
>Margon
Also you will need to lower the annealing temperature about 5 degrees or 
so because of the change in the salt conc. with the Pfu buffer!!!
-- 
Jody K. Hirsh
Northwestern University, Chicago, IL.   USA
jkh141 at nwu.edu




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