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PCR on Lambda

Institut fŸr Biochemie mfb102 at epix.uni-erlangen.de
Mon Nov 20 13:21:23 EST 1995

Hi Carl,
I have successfully amplified fragments out of two different lambda zap libraries
after diluting the stock 1:50 in water, freeze/thaw twice and using that as diluent
for the rest of the PCR reagents. However, if you do have a zap library you might
consider (suggestion from a colleague) to prepare a pool with the in vivo excision
protocol in order to get a better quality template which you can use more or less 
Cheers, Rainer Blaesius

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