I cloned some cosmids randomly in M13 to generate DNA sequencing, we shear
these cosmids by sonication and lately by nebulizing the DNA, afterwards we
made a DNA ends repair by using T4 DNA Pol. and Klenow enzyme and we separate
according to MW. by agarose gel. (~1100 bp.)
Our control is with lambda DNA digested with AluI enz.
My problem is the low efficiently of cloning after T4 and Klenow treatment.
Any suggestions are WELLCOME.