morten.lukacs at biokjemi.uio.no (Morten Lukacs) wrote:
>I need some info. about Bacterial alkaline phophatase (BAP)
I recommend shrimp alkaline phosphatase (SAP) as a simpler alternative to BAP.
SAP is active in most restriction enzyme buffers and can be inactivated by
heat, eliminating the need to extract your DNA sample after phosphatase
treatment. SAP is available from U.S. Biochemicals (no affiliation).
Thomas M. Duncan, Ph.D.
Dept. Biochemistry & Molecular Biology
SUNY Health Science Center at Syracuse
Email: duncant at vax.cs.hscsyr.edu or duncant at cross.bmb.hscsyr.edu