! Antibody purification

Mick Partis Mick.Partis at BBSRC.AC.UK
Tue Nov 21 03:55:32 EST 1995


Two further points to those already made.  Firstly, if there are a number 
of IgG binding proteins in your extract (not an uncommon occurence) you 
can remove the offending bands by pre-incubating the blot with an 
irrelevant IgG or serum, i.e. one from a species other than those from 
which your primary and secondary antibodies are obtained.  Secondly, it 
always pays not only to titrate your primary antibody and use it at the 
lowest concentration, but also the detecting antibody.

Mick




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