elebae at sara.cc.utu.fi wrote:
>Could somebody give me some advice concerning the Stratagene Pfu DNA
>polymerase(recombinant version)? I have tried quite many different PCR
>conditions, but still I don't get ANY product at all, not even non-specific!
Try using the native (non-recombinant) version of the enzyme which is also sold by Stratagene. I have found that native Pfu DNA polymerase will often amplify DNA that the recombinant version won't
Ion Channel Group
Centre for Mechanisms of Human Toxicity
University of Leicester, U.K.