Annealing temperature for oligos

Adela Torres Calatayud Adela.Torres at uv.es
Tue Nov 21 05:15:47 EST 1995


Hi everybody:

I'm currently working on DNA non-radioactive cycle sequencing, and I'd like to 
know if there is a reliable formula by which I can choose the annealing 
temperature of the PCR program when I'm sequencing using custom-made oligos. 
Right now the formula I'm using is:

		2(A+T) + 4(G+C) - 6 = T ann.

But it didn't give me good results when I tried it. So if there's anyone out 
there that can help me, thanks very much! E-mail or post.

					Adela Torres
				         (Adela.Torres at uv.es)



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