GIBCO M-MLv RT

[hfakhour]

Hi !

We recently changed from using a kit for reverse transcription into using GIBCO 
M-MLv reverse transcriptase. We have been trying to sort out a protocol for 
that, but so far no success.  The primers we use are random hexamers, our 
starting material is  total RNA (1-2 ug), we do not use any labelled dNTP. The 
resulting cDNA is used for PCR. Could anyone please suggest a succesful 
protocol.



Thanking you in advance

Hannah