GIBCO M-MLv RT

hfakhour hfakhour at fs1.ho.man.ac.uk
Tue Nov 21 13:42:22 EST 1995



Hi !

We recently changed from using a kit for reverse transcription into using GIBCO 
M-MLv reverse transcriptase. We have been trying to sort out a protocol for 
that, but so far no success.  The primers we use are random hexamers, our 
starting material is  total RNA (1-2 ug), we do not use any labelled dNTP. The 
resulting cDNA is used for PCR. Could anyone please suggest a succesful 
protocol.



Thanking you in advance

Hannah




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