Has onybody experience labeling gene probes by PCR and can provide
reaction conditions for this. It should also be possible to make single
stranded probes. If so, do you use a single stranded PCR product as
template or is a standard (double strand) PCR product as template fine.
Is the lower concentration of the 32P nucleotide a problem or do I have
to add cold nucleotide?
Any advice is welcome, thanks in advance