Probe labelling by PCR

[Thomas Urbig]

Has onybody experience labeling gene probes by PCR and can provide 
reaction conditions for this. It should also be possible to make single 
stranded probes. If so, do you use a single stranded PCR product as 
template or is a standard (double strand) PCR product as template fine. 
Is the lower concentration of the 32P nucleotide a problem or do I have 
to add cold nucleotide? 
Any advice is welcome, thanks in advance
  
               Thomas.