Probe labelling by PCR

Thomas Urbig Thomas at hastingslab.harvard.edu
Tue Nov 21 17:44:35 EST 1995


Has onybody experience labeling gene probes by PCR and can provide 
reaction conditions for this. It should also be possible to make single 
stranded probes. If so, do you use a single stranded PCR product as 
template or is a standard (double strand) PCR product as template fine. 
Is the lower concentration of the 32P nucleotide a problem or do I have 
to add cold nucleotide? 
Any advice is welcome, thanks in advance
  
               Thomas.





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