Probe labelling by PCR
Thomas at hastingslab.harvard.edu
Tue Nov 21 17:44:35 EST 1995
Has onybody experience labeling gene probes by PCR and can provide
reaction conditions for this. It should also be possible to make single
stranded probes. If so, do you use a single stranded PCR product as
template or is a standard (double strand) PCR product as template fine.
Is the lower concentration of the 32P nucleotide a problem or do I have
to add cold nucleotide?
Any advice is welcome, thanks in advance
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