bonhamp at duke.usask.ca (Peta C. Bonham-Smith) wrote:
>Has anyone been able to PCR amplify an insert directly from phage
>particles as oppposed to isolating phage DNA first? Do we need to treat
>the phage prior to PCR?
>University of Saskatchewan
>bonhamp at duke.usask.ca
We routinely use lambda lysates directly in pcr to subclone various
genes from the late gene region. The pcr products we are after are
usually .5 to 1.5 kb in size. The lysates range in titer from 5x10e8
to 1x10e10 and we do not pretreat the lysates in any way prior to pcr.
davesmith at bioch.tamu.edu