PCR from phage?

davesmith at bioch.tamu.edu davesmith at bioch.tamu.edu
Wed Nov 22 14:28:57 EST 1995


bonhamp at duke.usask.ca (Peta C. Bonham-Smith) wrote:

>Hi:
>Has anyone been able to PCR amplify an insert directly from phage 
>particles as oppposed to isolating phage DNA first?  Do we need to treat 
>the phage prior to PCR?

>Peta Bonham-Smith
>Assistant Professor
>University of Saskatchewan

>bonhamp at duke.usask.ca
  

We routinely use lambda lysates directly in pcr to subclone various
genes from the late gene region.  The pcr products we are after are
usually .5 to 1.5 kb in size.  The lysates range in titer from 5x10e8
to 1x10e10 and we do not pretreat the lysates in any way prior to pcr.
Good luck!
davesmith at bioch.tamu.edu




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