ASKING tips of mapping of methylated cytosines.

Karl Voss karl at hobbes.chem.ualberta.ca
Wed Nov 22 15:46:56 EST 1995


Constantin Polychronakos (mc97 at PO-Box.McGill.CA) wrote:
> Hi, does anyone can help me to solve the problem of 
> mapping of methylated cytosines.I exactly follow the paper of 
>  Nucleic Acids research 1994 Vol.22 No 15 Page 
> 2990-2997.  I cannot get PCR band after finishing the 
> convertion from C to U.

> Thanks
> lli, in LAb.

Make sure you get rid of all the bisulphite before doing the pcr.  Also
your template might be degraded depending on how carfully you controled the
conditions in the deamination reaction.

Karl

--

-------------------------------------------
Karl Voss
Department of Chemistry
University of Alberta
Edmonton, Alberta, Canada
T6G 2G2

karl at hobbes.chem.ualberta.ca
phone 403-492-0222
fax   403-492-8231



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