Help! Need some info on BAP!

Szatmari George szat at ERE.UMontreal.CA
Wed Nov 22 13:09:47 EST 1995


In article <48q0vn$oh0 at snowball.hscsyr.edu> Tom Duncan <duncant at cross.bmb.hscsyr.edu> writes:
>morten.lukacs at biokjemi.uio.no (Morten Lukacs) wrote:
>>I need some info. about Bacterial alkaline phophatase (BAP)
>>[...remainder deleted]
>
>	I recommend shrimp alkaline phosphatase (SAP) as a simpler alternative to BAP.
>SAP is active in most restriction enzyme buffers and can be inactivated by
>heat, eliminating the need to extract your DNA sample after phosphatase
>treatment. SAP is available from U.S. Biochemicals (no affiliation).
>	Cheers,
>
>	Tom.
>
>-- 
>	Thomas M. Duncan, Ph.D.
>	Dept. Biochemistry & Molecular Biology
>	SUNY Health Science Center at Syracuse
>	Email:	duncant at vax.cs.hscsyr.edu or duncant at cross.bmb.hscsyr.edu
>



I agree with Dr Duncan about the use of SAP vs BAP.  I just want to point
out that the supplier is now Amersham, who merged with USB last year.

George




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