How to make dimers or trimers by PCR?
tday at netcom.com
Wed Nov 22 13:31:35 EST 1995
I am using PCR to make linear copies of a plasmid for mutagenesis. I
would like to be able to make linear (head to tail) dimers or trimers, but
can't think of a way to do it (Ligation strongly favours
recircularisation). I don't want to make long multimers as this has
already been shown to introduce additional mutations.
Any ideas gratefully received.
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