How to make dimers or trimers by PCR?

Tony Day tday at netcom.com
Wed Nov 22 13:31:35 EST 1995


I am using PCR to make linear copies of a plasmid for mutagenesis.  I
would like to be able to make linear (head to tail) dimers or trimers, but
can't think of a way to do it (Ligation strongly favours
recircularisation).  I don't want to make long multimers as this has
already been shown to introduce additional mutations.

Any ideas gratefully received.

Thanks

Tony



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