At the moment I am designing primers for the NMDA receptor subunit 1 (or
zeta1 alt nomenclature). I am aware of the existance of to date 8 alt
splices. To begin, I want primers which recognize all forms ie "pan"
primers. However, I am having difficulty identifying which sequnences
are which in the EMBL genebank.
Could someone who works in this field give me an update on the matter. I
have looked at the recent TINS review (TINS Vol18, No.7 1995), but have
not succeeded in identifying which EMBL sequences correspond to the
different spliced isoforms.
I address this SOS to this group as the subject of PCR primers is
occasionally raised in this forum.
Thanks in anticipation,
ONXFOXXD at lgdx02.lg.ehu.es