linker annealing and clean-up

Francisco G da Nobrega ib - bio 7588 fgdnobre at usp.br
Thu Nov 23 08:14:08 EST 1995


Dear colleagues:

Is anyone familiar with the annealing conditions for 8-mers that=20
constitute a linker?=20
How one is supposed to clean-up the linkers before=20
use in ligation reactions?
How much to use in liagtion?=20
Will excess linkers inhibit the reaction?
Thanks for the feedback!

F.G. Nobrega
Universidade de S=E3o Paulo
Biologia - IBUSP
C.P.  11461
05422-970   S=E3o Paulo  -  SP  - Brasil
Voice: (011) 818-7588   Fax: (011) 818-7553    E-mail:  fgdnobre at usp.br




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