Measure E. coli density at O.D 600nm

Alex Chang lpss at
Thu Nov 23 20:32:47 EST 1995

Most people measure the density of E. coli at O.D. 600nm. I've also seen 
people measure it at 540nm.

The problem we have is that we only have a ELISA reader with UV lamps 
492nm and 620 nm which are closest to 600nm. I am wondering if that is ok 
to monitor the growth of my culture with one of them, and how that is 
compared with the results at 600nm.

Thanks very much.

Alex Chang
University of British Columbia
achang at

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