protease assay

[Pierce Chemical Co]

Protease activity can be measured by the Sorensen method.  It involves a change in amino 
groups detectable by TNBSA.  Succinylated casein is combined with the protease containing 
solution and as it is digested, new n-terminus amino groups are generated.  These can be 
compared with a standard curve generated with a known protease such as trypsin.
Tom Brotcke

Technical Assistance
Pierce Chemical Co
Phone: 1-815-968-0747   
Fax: 1-815-968-7316  
E-mail: PierceChem at mcimail.com