IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

apotosis assay

Shahram Mori smori at nmsu.edu
Tue Nov 21 23:48:05 EST 1995

ynakao at kean.ucs.mun.ca wrote:
: Hello, Netters.

: I am looking for the protocol for DNA fragmentation assay for detecting 
: Apotosis. Would you please tell me it or reference about it?

: Thanks in advance,

: Yoshi Nakao

In NAR there is a modification of an already existing technique called Random
Oligonucleotide Primed Synthesis (ROPS).
You clean up your DNA and generating a hot oligo using Klenow fragment.
Look at radioactivity incorporation -> Your assay.

Nucleic Acids Reserach Vol 22(13) 1994 pages 2714-2715.
			       _\  /_
			       \_  _/
			C   A   N   A   D   A
Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net