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Isolation of DNA

Shahram Mori smori at nmsu.edu
Tue Nov 21 23:38:37 EST 1995

Chris Wright (Wrightc2 at pilot.msu.edu) wrote:
: Does anyone out there have a good protocol for isolating genomic 
: eukaryotic cDNA from total community DNA which includes prokaryotes  
: (perhaps something involving pulse-field gel electrophoresis?).

You can't isolate cDNA from Eucaryotic cells......?
You have to isolate RNA from These Eucaryotic organisms and then make 
cDNA from them. The most common method is GITC and Phenol Extraction. 
Look in Analytical Biochemistry Vol 162:156 1987.

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Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM

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